![]() ![]() ![]() Note: The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition. Sizing of proteins on SDS-polyacrylamide gels and western blots.Monitoring protein transfer onto membranes after western blotting.Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.Membrane-compatible-colored bands transfer to membranes for Western blotting.Two reference bands-orange at 70 kDa and a mixture of blue and yellow ribbons at 10 kDa.Quality tested-each lot evaluated by SDS-PAGE and Western blotting.Sharp bands-color-coded bands of similar intensity for easy visualization.Ready-to-use-supplied in a loading buffer for direct loading on gels no need to boil.Size range-10 proteins spanning from 10 to 180 kDa. All western blot samples have three elements: protein extract, cell lysis buffer, and Laemmli (sample) buffer.The ladder contains one orange reference band at 70 kDa and a mixture of blue and yellow ribbons at 10 kDa. This prestained protein marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Immun-Blot PVDF membrane retains proteins in any transfer format tank blotting, semi-dry blotting, and. The absence of the signal during ponceau staining is not a very good indicator because any staining procedure is much less sensitive than immunodetection. ABP Prestained Protein Marker is a mixture of ten blue, orange or green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein marker is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use. Immun-Blot PVDF Membrane is ideal for chemiluminescent and colorimetric western blots because it retains target protein very strongly, 140150 µg pro-tein/cm2 membrane, but resists background that can obscure high sensitivity detection. If you see the ladder markers on your membrane but don't see any bands of your protein it means there is something wrong with your 1st or 2nd antibodies steps. ![]()
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